Nepal National Sero-Prevalence Survey for COVID-19 2020 Round 1

A multistage cluster sampling method was used to select a representative sample of the population. The administrative wards were considered as clusters, and 30 were selected from each province. In each cluster, 200-250 households were expected to be included. Then fifteen households were randomly selected from each selected cluster (or the segment) for inclusion in the survey. One eligible member of the selected household who was residing in Nepal for at least four weeks prior to the survey date was enrolled by a random selection. The eligible age limit was defined as six months or older. The estimated sample size for this survey to obtain a precise estimate of national level Seroprevalence was 3,150. The samples were tested for COVID-19 total antibody presence using WHO-approved WANTAI SARS-CoV-2 antibody ELISA kits. This ELISA test detects both IgM and IgG antibodies for SARSCoV-2 virus.